GA黄金甲

为了更好地相识人类分歧种群的遗传差距和受到的天然选择情况，以及比力分歧指标之间的印证关系。近日，GA黄金甲曾长青钻研员尝试室的程锋等人，通过选择目前SNP（单核苷酸多态性）分型数量最大和种群数最多的HapMap（国际人类基因组单体型图打算）分型数据作为钻研基础，从基因组大片段，职能基因以及单个SNP位点等三个档次来钻研人类分歧种群的基因组遗传分化和所受天然选择的情况。凭据使用多个分歧指标（HET，Win_HET, FST, Win_FST, iHS, ES_HET, ES_FST, P_iHS等）及战术来扫描选择信号，并把它们置于统一个框架下进行比力和验证，以求获得最大的信息。钻研了局成立了“人类多档次多人群天然选择数据库”暨阳性天然选择数据库SNP@Evolution (http://bighapmap.big.ac.cn/)供国内表科研使用，自九月下旬有关文章在BMC Evol Biol颁发以来，SNP@Evolution已受到来自全世界几十个国度和地域，上万次的接见和下载，为该领域的钻研人员提供了一个发现选择信号的有效工具。

SNP@Evolution共分为数据查问和图形查问界面两个部门。蕴含了HapMap II期和III期的数据了局。II期共有3,619,226个SNP数据，以及21,859个基因的分析数据。共有1606个基因组大片段显示选择信号，660个显示分化信号。III期数据共蕴含1,389,498 SNPs， 21,099个有效基因分析数据。在11幼我群中找到了10,138个受选择的基因组片段，以及464个拥有强分化的基因组片段。为了方便钻研，SNP@Evolution的查问了局能够链接到其他数据库获取更多信息。 

数据库链接:

http://bighapmap.big.ac.cn

文件纪录:

Cheng Feng, Chen Wei, Richards Elliott, Deng Libin, Zeng Changqing. SNP@Evolution: a hierarchical database of positive selection on the human genome. BMC Evolutionary Biology 2009, 9:221.

原文链接:

http://www.biomedcentral.com/1471-2148/9/221

原文提要:

Abstract

Background: Positive selection is a driving force that has shaped the modern human. Recent developments in high throughput technologies and corresponding statistics tools have made it possible to conduct whole genome surveys at a population scale, and a variety of measurements, such as heterozygosity (HET), FST, and Tajima's D, have been applied to multiple datasets to identify signals of positive selection. However, great effort has been required to combine various types of data from individual sources, and incompatibility among datasets has been a common problem. SNP@Evolution, a new database which integrates multiple datasets, will greatly assist future work in this area.

Description: As part of our research scanning for evolutionary signals in HapMap Phase II and Phase III datasets, we built SNP@Evolution as a multi-aspect database focused on positive selection. Among its many features, SNP@Evolution provides computed FST and HET of all HapMap SNPs, 5+ HapMap SNPs per qualified gene, and all autosome regions detected from whole genome window scanning. In an attempt to capture multiple selection signals across the genome, selection-signal enrichment strength (ES) values of HET, FST, and P-values of iHS of most annotated genes have been calculated and integrated within one frame for users to search for outliers. Genes with significant ES or P-values (with thresholds of 0.95 and 0.05, respectively) have been highlighted in color. Low diversity chromosome regions have been detected by sliding a 100 kb window in a 10 kb step. To allow this information to be easily disseminated, a graphical user interface (GBrowser) was constructed with the Generic Model Organism Database toolkit.

Conclusion: Available at http://bighapmap.big.ac.cn, SNP@Evolution is a hierarchical database focused on positive selection of the human genome. Based on HapMap Phase II and III data, SNP@Evolution includes 3,619,226/1,389,498 SNPs with their computed HET and FST, as well as qualified genes of 21,859/21,099 with ES values of HET and FST. In at least one HapMap population group, window scanning for selection signals has resulted in 1,606/10,138 large low HET regions. Among Phase II and III geographical groups, 660 and 464 regions show strong differentiation.